immunoassay
3 interactions related to immunoassay
levothyroxine + biotin
High-dose biotin (B7) does not directly interact with levothyroxine pharmacologically, but it interferes with biotin-streptavidin immunoassays used for TSH, free T4, free T3, and thyroglobulin. This can produce falsely low TSH and falsely high T4/T3, mimicking hyperthyroidism and leading to inappropriate dose reductions.
biotin + troponin test
High-dose biotin (vitamin B7) interferes with biotin-streptavidin-based immunoassays used to measure cardiac troponin, producing falsely low results that can mask an evolving heart attack. The FDA has received reports of patient harm, including one death linked to a missed myocardial infarction diagnosis caused by biotin-altered troponin readings.
biotin + thyroid stimulating hormone test
High-dose biotin interferes with biotin-streptavidin-based immunoassays used to measure TSH, free T3, free T4, and thyroglobulin, producing a falsely low TSH and falsely elevated free thyroid hormones, a pattern that mimics Graves' disease. Patients have been incorrectly diagnosed with hyperthyroidism and started on antithyroid drugs because of biotin interference.